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磷脂酰肌醇 3-激酶在微藻油脂代谢中的功能研究
发布时间:2020
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论文题目 Transcriptional reprogramming of intermediate metabolism gene induced by Phosphatidylinositol 3-Kinase in Phaeodactylum tricnutum
作者姓名 张惠莹,殷炜铧,马栋,包倩,刘鑫,徐开放,赵超,缪颖,刘建
是否发表 刊 号 2211-9264
期刊名称 期刊期次 47
项目名称 磷脂酰肌醇 3-激酶在微藻油脂代谢中的功能研究
项目类型 创新训练项目
项目编号 202010389025 学科类别 工学——能源动力类
立项年份 2020 联系QQ号 1439711624
项目组成员 姓 名 年 级 专 业 是否主持人
殷炜铧2017级生物信息学(1)班第一主持人
徐梦雪2019级生物科学(2)班
杨倩2019级生物科学(1)班
董广辉2019级生物科学(2)班
肖家旺2019级生物科学(生物学基地班)
指导老师 姓 名 职称 研究方向
张惠莹 副教授 应用藻类学、基因工程
刘 建 讲师 细胞信号传导、分子生物学
论文摘要 Phosphatidylinositol 3-kinase and its product phosphatidylinositol 3-phosphate play a key role in phospholipid and energy metabolism, protein traffic and exocytosis. However, the role of phosphatidylinositol 3-kinase on lipid production in Phaeodactylum tricornutum is still unclear. Phosphatidylinositol 3-kinase in Phaeodactylum tricornutum is more closely associated with homologs from mammals than higher plants. Here, we use pharmacological, transcriptome, and biochemical approaches to reveal that phosphatidylinositol 3-kinase signaling was involved in lipid accumulation. For investigating the role of phosphatidylinositol 3-kinase in Phaeodactylum tricornutum, phosphatidylinositol 3-kinase inhibitors, wortmannin and LY294002, were used. Fluorescein dia-cetate fluorescence analysis showed that 1 uM wortmannin and 1 uM LY294002 had no significant effect on cellular viability. Treatment with phosphatidylinositol 3-kinase inhibitors increased triacylglycerols production and the ratio of saturation fatty acid but extended the logarithmic phase of the cells. Metabolite content analysis showed that total content of soluble proteins was decreased, whereas the total lipids content and carbohydrates content were remarkably increased under phosphatidylinositol 3-kinase inhibitors treatment. RNA-Seq analysisshowed the expression of genes involved in starch and sucrose metabolism, nitrogen and amino acid metabolism were upregulated while that in pigment metabolism, RNA metabolism were downregulated under wortmannin treatment in standard condition. The pathway induced by wortmannin under nitrogen deprivation conditionsseems to be different in standard conditions. The expression of gene involved in RNA metabolism and MAPK signaling was upregulated while that in lipid metabolism was decreased under wortmannin treatment in N-freeculture. This study demonstrated a role of phosphatidylinositol 3-kinase in the allocation of carbon and reductant towards lipids in Phaeodactylum tricornutum.

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